[Histonet] histoclear vs xylene

[Campbell, John Thomas (UMR-Student)]

I have read several protocols on staining slides and most of them talk about using xylene the protocol that I am currently using says to use Histoclear. I was wondering what the difference was and I would like to know the pros and cons on using xylene and using histoclear. what do you all use and what exactly are the hazards of using xylene

John Campbell
Graduate Student
University of Missouri Rolla
Lab phone [phone]
Cell phone [phone]

[Rene J Buesa]

John:
In a "nutshell" I can tell you the following:
1-xylene: universally used for tissue processing and staining but a product that should be avoided because of known health hazards.
2-histoclear: a "xylene substitute" sometimes used as a replacement of xylene. Does not work equally well. It is "allegedly safe" but really with no documentation of long term health effects as xylene has. Maybe with time it will turn out to be as hazardous as xylene.
Our profession has some health hazards and we just have to work as safely as possible.
Hope this will help you!

"Campbell, John Thomas (UMR-Student)" <***@umr.edu> wrote:
I have read several protocols on staining slides and most of them talk about using xylene the protocol that I am currently using says to use Histoclear. I was wondering what the difference was and I would like to know the pros and cons on using xylene and using histoclear. what do you all use and what exactly are the hazards of using xylene

John Campbell
Graduate Student
University of Missouri Rolla
Lab phone [phone]
Cell phone [phone]


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[Bruce Abaloz]

John,
I agree with Rene that Histoclear doesn't work as well as Xylene.
We used to deparrafinize routinely in Xylene...X2 changes, 3 min.
each.
Now we use Histolene/Histoclear, x2 changes of 10 min. each - a
third wd. probably be good (?) We now have gone back to
deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as
were having problems with 'end results', until - we went back to
deparrafinizing in Xylene!
As far as the "known health hazards" - check your MSDS/Xylene; it
is not even considered Toxic....simply make sure you do all your
work under a fumehood/be sensible...I use it to purge my Tissue
Processer as - NOTHING works like Xylene.

Cheers,
Bruce in OZ

1-xylene: universally used for tissue processing and staining but
a product that should be avoided because of known health hazards.
2-histoclear: a "xylene substitute" sometimes used as a
replacement of xylene. Does not work equally well. It is "allegedly
safe" but really with no documentation of long term health effects
as xylene has. Maybe with time it will turn out to be as hazardous
as xylene.
Our profession has some health hazards and we just have to work as
safely as possible.
Hope this will help you!
John Campbell
Graduate Student
University of Missouri Rolla
Lab phone [phone]
Cell phone [phone]
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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Next-gen email? Have it all with the all-new Yahoo! Mail Beta.
_______________________________________________
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--
BRUCE ABALOZ
PH:61383446282
HISTOLOGIST
FAX:[phone]
DEPT. of ZOOLOGY
EMAIL: ***@unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
Nobody Can Make You Feel Inferior Without YOUR Permission -
Eleanor Roosevelt
DANCE LIKE
NO-ONE'S WATCHING
Q: What's a specimen?
A: An Italian astronaut.

[Favara, Cynthia (NIH/NIAID) [E]]

Here is the web site on xylenes from the EPA

http://www.epa.gov/ttn/atw/hlthef/xylenes.html

I would not necessarily agree that "it is not even considered Toxic" but
I do agree nothing is quite like xylene or toluene for histo.

c


Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Bruce Abaloz [mailto:***@unimelb.edu.au]
Sent: Wednesday, June 21, 2006 5:28 PM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] histoclear vs xylene

John,
I agree with Rene that Histoclear doesn't work as well as Xylene.
We used to deparrafinize routinely in Xylene...X2 changes, 3 min.
each.
Now we use Histolene/Histoclear, x2 changes of 10 min. each - a
third wd. probably be good (?) We now have gone back to
deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as
were having problems with 'end results', until - we went back to
deparrafinizing in Xylene!
As far as the "known health hazards" - check your MSDS/Xylene; it
is not even considered Toxic....simply make sure you do all your
work under a fumehood/be sensible...I use it to purge my Tissue
Processer as - NOTHING works like Xylene.

Cheers,
Bruce in OZ

1-xylene: universally used for tissue processing and staining but
a product that should be avoided because of known health hazards.
2-histoclear: a "xylene substitute" sometimes used as a
replacement of xylene. Does not work equally well. It is "allegedly
safe" but really with no documentation of long term health effects
as xylene has. Maybe with time it will turn out to be as hazardous
as xylene.
Our profession has some health hazards and we just have to work as
safely as possible.
Hope this will help you!
John Campbell
Graduate Student
University of Missouri Rolla
Lab phone [phone]
Cell phone [phone]
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Do you Yahoo!?
Next-gen email? Have it all with the all-new Yahoo! Mail Beta.
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

--
BRUCE ABALOZ
PH:61383446282
HISTOLOGIST
FAX:[phone]
DEPT. of ZOOLOGY
EMAIL: ***@unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
Nobody Can Make You Feel Inferior Without YOUR Permission -
Eleanor Roosevelt
DANCE LIKE
NO-ONE'S WATCHING
Q: What's a specimen?
A: An Italian astronaut.

[Anne Van Binsbergen]

ozziebruce
'not considered as toxic'- this level of ignorance NEVER fails to amaze
me.....perhaps you need to do a little more reading up on xylene and
associated harmful effects - there are many!!!!
annieinarabia

-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Bruce
Abaloz
Sent: Thursday, June 22, 2006 4:28 AM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] histoclear vs xylene

John,
I agree with Rene that Histoclear doesn't work as well as Xylene.
We used to deparrafinize routinely in Xylene...X2 changes, 3 min.
each.
Now we use Histolene/Histoclear, x2 changes of 10 min. each - a
third wd. probably be good (?) We now have gone back to
deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as
were having problems with 'end results', until - we went back to
deparrafinizing in Xylene!
As far as the "known health hazards" - check your MSDS/Xylene; it
is not even considered Toxic....simply make sure you do all your
work under a fumehood/be sensible...I use it to purge my Tissue
Processer as - NOTHING works like Xylene.

Cheers,
Bruce in OZ

1-xylene: universally used for tissue processing and staining but
a product that should be avoided because of known health hazards.
2-histoclear: a "xylene substitute" sometimes used as a
replacement of xylene. Does not work equally well. It is "allegedly
safe" but really with no documentation of long term health effects
as xylene has. Maybe with time it will turn out to be as hazardous
as xylene.
Our profession has some health hazards and we just have to work as
safely as possible.
Hope this will help you!
John Campbell
Graduate Student
University of Missouri Rolla
Lab phone [phone]
Cell phone [phone]
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Do you Yahoo!?
Next-gen email? Have it all with the all-new Yahoo! Mail Beta.
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

--
BRUCE ABALOZ
PH:61383446282
HISTOLOGIST
FAX:[phone]
DEPT. of ZOOLOGY
EMAIL: ***@unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
Nobody Can Make You Feel Inferior Without YOUR Permission -
Eleanor Roosevelt
DANCE LIKE
NO-ONE'S WATCHING
Q: What's a specimen?
A: An Italian astronaut.

[Smith, Allen]

Although we carefully save our MSDS's in a neat alphabetical file, what we
actually consult is Lewis and Sax's HAZARDOUS CHEMICALS DESK REFERENCE (kept
in my office) or HAZARDOUS PROPERTIES OF INDUSTRIAL MATERIALS (kept in the
lab). Lewis and Sax say of xylene: "Moderately toxic by inhalation,
ingestion, and subcutaneous routes... Irritation can start at 200 ppm."
Bruce, however is taking due precautions by using xylene in a fume hood.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161


-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Anne Van
Binsbergen
Sent: Thursday, June 22, 2006 6:06 AM
To: Bruce Abaloz; ***@lists.utsouthwestern.edu
Subject: RE: [Histonet] histoclear vs xylene

ozziebruce
'not considered as toxic'- this level of ignorance NEVER fails to amaze
me.....perhaps you need to do a little more reading up on xylene and
associated harmful effects - there are many!!!!
annieinarabia

-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Bruce
Abaloz
Sent: Thursday, June 22, 2006 4:28 AM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] histoclear vs xylene

John,
I agree with Rene that Histoclear doesn't work as well as Xylene.
We used to deparrafinize routinely in Xylene...X2 changes, 3 min.
each.
Now we use Histolene/Histoclear, x2 changes of 10 min. each - a
third wd. probably be good (?) We now have gone back to
deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as
were having problems with 'end results', until - we went back to
deparrafinizing in Xylene!
As far as the "known health hazards" - check your MSDS/Xylene; it
is not even considered Toxic....simply make sure you do all your
work under a fumehood/be sensible...I use it to purge my Tissue
Processer as - NOTHING works like Xylene.

Cheers,
Bruce in OZ

1-xylene: universally used for tissue processing and staining but
a product that should be avoided because of known health hazards.
2-histoclear: a "xylene substitute" sometimes used as a
replacement of xylene. Does not work equally well. It is "allegedly
safe" but really with no documentation of long term health effects
as xylene has. Maybe with time it will turn out to be as hazardous
as xylene.
Our profession has some health hazards and we just have to work as
safely as possible.
Hope this will help you!
John Campbell
Graduate Student
University of Missouri Rolla
Lab phone [phone]
Cell phone [phone]
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
Do you Yahoo!?
Next-gen email? Have it all with the all-new Yahoo! Mail Beta.
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

--
BRUCE ABALOZ
PH:61383446282
HISTOLOGIST
FAX:[phone]
DEPT. of ZOOLOGY
EMAIL: ***@unimelb.edu.au
THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010
Nobody Can Make You Feel Inferior Without YOUR Permission -
Eleanor Roosevelt
DANCE LIKE
NO-ONE'S WATCHING
Q: What's a specimen?
A: An Italian astronaut.
_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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