Discussion:
Ventana antibody - H pylori
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Histology Technician
2015-07-09 20:51:12 UTC
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Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain?  My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved.  Any thoughts?  Thanks!
Cartun, Richard
2015-07-09 21:05:51 UTC
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Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Histology Technician [mailto:***@att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-***@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks!
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Sebree Linda A
2015-07-09 21:16:09 UTC
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Rabbit monoclonal, Richard.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Cartun, Richard [mailto:***@hhchealth.org]
Sent: Thursday, July 09, 2015 4:06 PM
To: Histology Technician; Histonet; histonet-***@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Histology Technician [mailto:***@att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-***@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks!
_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
Susan Foreman
2015-07-10 12:07:59 UTC
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We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra
using UltraView DAB detection. He have had dirty staining for spirochetes
(syphilis) in the past utilizing the Red Detection kit and decons do seem to
help a bit. The DAB detection kit is much cleaner.

-----Original Message-----
From: Sebree Linda A [mailto:***@uwhealth.org]
Sent: Thursday, July 09, 2015 5:16 PM
To: 'Cartun, Richard'; Histology Technician; Histonet;
histonet-***@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Rabbit monoclonal, Richard.

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Cartun, Richard [mailto:***@hhchealth.org]
Sent: Thursday, July 09, 2015 4:06 PM
To: Histology Technician; Histonet;
histonet-***@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Histology Technician [mailto:***@att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-***@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

Does anyone else have constant problems with the Ventana antibody H pylori
having a dirty background stain? My pathologists are constantly complaining
about it and when I call CS I get the same response...decon the instrument,
try a new lot number, etc... it's never truly resolved. Any thoughts?
Thanks!
_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential and privileged
information. Any unauthorized review, use, disclosure, or distribution is
prohibited. If you are not the intended recipient, or an employee or agent
responsible for delivering the message to the intended recipient, please
contact the sender by reply e-mail and destroy all copies of the original
message, including any attachments.
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Dyer, Terry
2015-07-10 20:15:30 UTC
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Happy Friday1
We are experiencing the same problem. Just had a site visit from Ventana and they suggested using an antibody dilution buffer. Use an option kit filled with Ventana Product ADB250 (antibody dilution buffer). Check options in protocol editing and 8 minutes, suggested time. Have not tried it but inted to. Good luck.

-----Original Message-----
From: Susan Foreman [mailto:***@labpath.com]
Sent: Friday, July 10, 2015 5:08 AM
To: 'Sebree Linda A'; 'Cartun, Richard'; 'Histology Technician'; 'Histonet'; histonet-***@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra using UltraView DAB detection. He have had dirty staining for spirochetes
(syphilis) in the past utilizing the Red Detection kit and decons do seem to help a bit. The DAB detection kit is much cleaner.

-----Original Message-----
From: Sebree Linda A [mailto:***@uwhealth.org]
Sent: Thursday, July 09, 2015 5:16 PM
To: 'Cartun, Richard'; Histology Technician; Histonet; histonet-***@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Rabbit monoclonal, Richard.

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

-----Original Message-----
From: Cartun, Richard [mailto:***@hhchealth.org]
Sent: Thursday, July 09, 2015 4:06 PM
To: Histology Technician; Histonet;
histonet-***@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Histology Technician [mailto:***@att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-***@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts?
Thanks!
_______________________________________________
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Sebree Linda A
2015-07-09 21:17:28 UTC
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We have excellent and very clean results with the VMS H. Pylori on our Ultras. I can shoot you our protocol if you're interested.

Linda

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Histology Technician [mailto:***@att.net]
Sent: Thursday, July 09, 2015 3:51 PM
To: Histonet; histonet-***@lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain?  My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved.  Any thoughts?  Thanks!
_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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