Discussion:
National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
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Adesupo, Adesuyi (Banjo)
2015-07-13 16:52:29 UTC
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Hi,
I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning.
I wanted to know whether some of you guys out there are using Formaldehyde substitute.


Best regards,

Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
Histology Supervisor
Norman Regional Health System,
Norman, OK 73071.
Tel: 405- 307- 1145
***@nrh-ok.com<mailto:***@nrh-ok.com>

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Stedman, Nancy
2015-07-13 19:12:21 UTC
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We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons.

Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world.

-Nancy


Nancy L. Stedman DVM PhD Dipl ACVP
Veterinary Pathologist
Busch Gardens Tampa
***@buschgardens.com



-----Original Message-----
From: Adesupo, Adesuyi (Banjo) [mailto:***@nrh-ok.com]
Sent: Monday, July 13, 2015 12:52 PM
To: '***@lists.utsouthwestern.edu'
Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

Hi,
I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning.
I wanted to know whether some of you guys out there are using Formaldehyde substitute.


Best regards,

Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
Histology Supervisor
Norman Regional Health System,
Norman, OK 73071.
Tel: 405- 307- 1145
***@nrh-ok.com<mailto:***@nrh-ok.com>

======================================
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments.
Weems, Joyce K.
2015-07-13 20:09:44 UTC
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The problem in the clinical world is that many clinical trials require formalin fixation, so I would not want to substitute anything that would prevent a patient from being in a trial.

It's not new to me that formalin is a carcinogen. I just try to make sure to use PPE and have good ventilation and monitor as necessary. (Unlike when I put my ungloved hands in it and had no ventilation when I started down this path a hundred years ago.) j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
***@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.


-----Original Message-----
From: Stedman, Nancy [mailto:***@buschgardens.com]
Sent: Monday, July 13, 2015 3:12 PM
To: Adesupo, Adesuyi (Banjo); '***@lists.utsouthwestern.edu'
Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons.

Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world.

-Nancy


Nancy L. Stedman DVM PhD Dipl ACVP
Veterinary Pathologist
Busch Gardens Tampa
***@buschgardens.com



-----Original Message-----
From: Adesupo, Adesuyi (Banjo) [mailto:***@nrh-ok.com]
Sent: Monday, July 13, 2015 12:52 PM
To: '***@lists.utsouthwestern.edu'
Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

Hi,
I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning.
I wanted to know whether some of you guys out there are using Formaldehyde substitute.


Best regards,

Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
Histology Supervisor
Norman Regional Health System,
Norman, OK 73071.
Tel: 405- 307- 1145
***@nrh-ok.com<mailto:***@nrh-ok.com>

======================================
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments.
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Paula Pierce
2015-07-13 20:42:22 UTC
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Like and Ditto to Joyce. Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH  405-759-7513 FAX www.excaliburpathology.com
From: "Weems, Joyce K." <***@emoryhealthcare.org>
To: "Stedman, Nancy" <***@buschgardens.com>; "Adesupo, Adesuyi (Banjo)" <***@nrh-ok.com>; "'***@lists.utsouthwestern.edu'" <***@lists.utsouthwestern.edu>
Sent: Monday, July 13, 2015 3:09 PM
Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

The problem in the clinical world is that many clinical trials require formalin fixation, so I would not want to substitute anything that would prevent a patient from being in a trial.

It's not new to me that formalin is a carcinogen. I just try to make sure to use PPE and have good ventilation and monitor as necessary. (Unlike when I put my ungloved hands in it and had no ventilation when I started down this path a hundred years ago.)  j

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
***@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.


-----Original Message-----
From: Stedman, Nancy [mailto:***@buschgardens.com]
Sent: Monday, July 13, 2015 3:12 PM
To: Adesupo, Adesuyi (Banjo); '***@lists.utsouthwestern.edu'
Subject: Re: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

We are using Excell Plus made by American Master Tech.  It is a combination of alcohol, ethylene glycol, and glyoxal.  It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty.  We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus.  It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection.  We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues.  We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus.  GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons.

Hope this is helpful.  I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world.

-Nancy


Nancy L. Stedman DVM PhD Dipl ACVP
Veterinary Pathologist
Busch Gardens Tampa
***@buschgardens.com



-----Original Message-----
From: Adesupo, Adesuyi (Banjo) [mailto:***@nrh-ok.com]
Sent: Monday, July 13, 2015 12:52 PM
To: '***@lists.utsouthwestern.edu'
Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories

    Hi,
      I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning.
    I wanted to know whether some of you guys out there are using Formaldehyde substitute.


    Best regards,

  Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145
  ***@nrh-ok.com<mailto:***@nrh-ok.com>

======================================
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments.
_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
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________________________________

This e-mail message (including any attachments) is for the sole use of
the intended recipient(s) and may contain confidential and privileged
information. If the reader of this message is not the intended
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or copying of this message (including any attachments) is strictly
prohibited.

If you have received this message in error, please contact
the sender by reply e-mail message and destroy all copies of the
original message (including attachments).



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