Discussion:
1. Re: Coverslipping mystery (Caroline Miller)
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Vontell, Regina
2015-07-12 15:21:01 UTC
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1. Re: Coverslipping mystery

I agree with Carol, my lab uses DPX and I have not had a problem with the product drying out. I do notice that if air gets trapped in the viscous solution there is a potential for the coverslips to fall off or have large air pockets.
We use wooden chopsticks to apply the DPX to the slide. I find this works really well and it reduces the amount of air bubbles that form with using a plastic squeeze pipette.
Regina
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Today's Topics:

1. Re: Coverslipping mystery (Caroline Miller)


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Message: 1
Date: Sat, 11 Jul 2015 07:17:30 -0700
From: Caroline Miller <***@3scan.com>
To: John Kiernan <***@uwo.ca>
Cc: Adam Boanas <***@epistem.co.uk>,
"***@lists.utsouthwestern.edu"
<***@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Coverslipping mystery
Message-ID: <6C960C7A-BC93-463A-A408-***@3scan.com>
Content-Type: text/plain; charset=us-ascii

I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time.....

When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something!

Yours,
mills

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media.
In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant.
Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips.
John Kiernan
= = =
Hello,
We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance wh!
en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it?
We are struggling for ideas with this one! - this occurs with fresh DPX also.
Many thanks
Adam
Adam Boanas
Senior Research Associate
Epistem Ltd
48 Grafton Street
Manchester, M13 9XX
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D***@hattiesburgclinic.com
2015-07-13 11:41:55 UTC
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I'm studying for the ASCP Safety Qualification. Looking for advice from an experienced source. Thanks!


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Today's Topics:

1. Re: Coverslipping mystery (Caroline Miller)


----------------------------------------------------------------------

Message: 1
Date: Sat, 11 Jul 2015 07:17:30 -0700
From: Caroline Miller <***@3scan.com>
To: John Kiernan <***@uwo.ca>
Cc: Adam Boanas <***@epistem.co.uk>,
"***@lists.utsouthwestern.edu"
<***@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Coverslipping mystery
Message-ID: <6C960C7A-BC93-463A-A408-***@3scan.com>
Content-Type: text/plain; charset=us-ascii

I really like DPX, although funnily enough we used cytoseal in my lab in London but always called it the DPX! I think I remember by boss telling me about the bad DPX time.....

When I moved to the USA the lab I started in had a bottle of DPX and i loved it! I always decant some of the DPX into a 100ml glass bottle, put in a plastic squeeze pipette and then screw a lid on it to stop it drying out (with the pipette still inside) when not in use. Surprisingly the pipette doesn't melt! Which is good because I am a recycle freak and i couldn't stand using a new one every time I mounted something!

Yours,
mills

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297
DPX is a polystyrene mounting medium. In principle you can make your own from published recipes. In practice, everyone buys commercial resinous mounting media.
In the 1990s we had trouble similar to what you describe. The commercial DPX was cloudy, and not because of alcohol in our xylene. The Canadian supplier acknowledged the bad DPX and urged us to buy Entellan instead. Entellan is a poly(methacrylate) plastic and is an excellent but expensive mounting medium. Another poly(methacrylate) mountant called CytoSeal was less expensive and also came in a squeeze-easy plastic bottle for delivery onto the slide or coverslip. It's now my routine resious mountant.
Good DPX returned to the market in the 2000s, but in old-fashioned bottles and not easy to apply to slides or coverslips.
John Kiernan
= = =
Hello,
We are having a problem that is developing into a big issue in our lab and I was wondering if anybody could shed any light on it. Our CV5000 coverslipper has recently started introducing microscopic air bubbles onto the slides during coverslipping. We have been told by our engineer that it is a consequence of the age and use of the motor and that sourcing another for an instrument that old (15yrs) will be v difficult. As such, we have been forced to manually coverslip using DPX and a pipette - manually applying the coverslips to the slide, thus mirroring the action of the coverslipper. This is fine at first and for the next few days the slides look great and very clean. However, after about day 4 -5 days post coverslipping, the slides develop an odd appearance down the microscope which looks like very fine `parched earth / crazy paving` all over the slide - including the section. The excess mountant around the edge of the coverslip also has a very faint, cloudy appearance
wh!
en this occurs. This of course renders the slide un-useable. Does anyone have a clue what this might be down to / how we can stop it?
We are struggling for ideas with this one! - this occurs with fresh DPX also.
Many thanks
Adam
Adam Boanas
Senior Research Associate
Epistem Ltd
48 Grafton Street
Manchester, M13 9XX
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------

Subject: Digest Footer

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------------------------------

End of Histonet Digest, Vol 140, Issue 12
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