[Histonet] aqueous mounting medium nad refractive index

[David Laidley]

Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums.

Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available)

David Laidley
Memorial University of Newfoundland
Division of Basic Medical Sciences
St. John's, Newfoundland

***@yahoo.ca




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[Monson, Frederick]

Hi Dave,
Pardon the duplication, but an answer is most efficient on a list,
but attachments are not.

I have sent you a PDF file of a Refractive Index summary that I put
together at the beginning of 2003. Any others who would like it should
request it of me by off-list email using:

Subject: RI

No text will be required - nor thanks since I will assume each email so
received to include all that is required for me to quickly respond.

Cheers,

Fred Monson

Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone/FAX: [phone]
eMail: ***@wcupa.edu
CASI Page and Scheduling
http://darwin.wcupa.edu/CASI/


-----Original Message-----
From: David Laidley [mailto:***@yahoo.ca]
Sent: Tuesday, December 02, 2003 12:21 PM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] aqueous mounting medium nad refractive index


Does anyone know the refractive index of Faramount (Dako) and
PVA-glycerol, both aqueous mounting mediums.

Can anyone suggest an aqueous mounting medium that has a better
refractive index than the ones mentioned above (if available)

David Laidley
Memorial University of Newfoundland
Division of Basic Medical Sciences
St. John's, Newfoundland

***@yahoo.ca




_____

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[Barry R Rittman]

David
If "faramount" is the same as Farrant's mountant it is around 1.425.
PVA mountants can have a RI starting at approximately 1.3 and Gray's
formula has a RI of 1.382
Glycerol has RI of 1.313 to 1.47 depending on its concentration (78 ml.
glycerin + 22 ml. of water RI = 1.44, 1:1 glycerin:water has a RI of
1.4).
There are a large number of formulae .
What specifically were you interested in placing in an aqueous mountant?
Barry

-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of David
Laidley
Sent: Tuesday, December 02, 2003 11:21 AM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] aqueous mounting medium nad refractive index


Does anyone know the refractive index of Faramount (Dako) and
PVA-glycerol, both aqueous mounting mediums.

Can anyone suggest an aqueous mounting medium that has a better
refractive index than the ones mentioned above (if available)

David Laidley
Memorial University of Newfoundland
Division of Basic Medical Sciences
St. John's, Newfoundland

***@yahoo.ca





Post your free ad now! Yahoo! Canada Personals

[Morken, Tim - Labvision]

Barry, It's not the same. "Faramount" is a DAKO product named after a person
named Faranak, who worked at DAKO and developed it. I believe it is now
called DAKOmount since Faranak left the company.

Tim Morken
Lab Vision / NeoMarkers
email: ***@labvision.com
www.labvision.com


-----Original Message-----
From: Barry R Rittman [mailto:***@uth.tmc.edu]
Sent: Tuesday, December 02, 2003 10:03 AM
To: ***@lists.utsouthwestern.edu
Subject: RE: [Histonet] aqueous mounting medium nad refractive index

David
If "faramount" is the same as Farrant's mountant it is around 1.425.
PVA mountants can have a RI starting at approximately 1.3 and Gray's
formula has a RI of 1.382
Glycerol has RI of 1.313 to 1.47 depending on its concentration (78 ml.
glycerin + 22 ml. of water RI = 1.44, 1:1 glycerin:water has a RI of
1.4).
There are a large number of formulae .
What specifically were you interested in placing in an aqueous mountant?
Barry

-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of David
Laidley
Sent: Tuesday, December 02, 2003 11:21 AM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] aqueous mounting medium nad refractive index


Does anyone know the refractive index of Faramount (Dako) and
PVA-glycerol, both aqueous mounting mediums.

Can anyone suggest an aqueous mounting medium that has a better
refractive index than the ones mentioned above (if available)

David Laidley
Memorial University of Newfoundland
Division of Basic Medical Sciences
St. John's, Newfoundland

***@yahoo.ca





Post your free ad now! Yahoo! Canada Personals

[Bryan Llewellyn]

By better refractive index, I presume you mean higher refractive index. If that is correct, the aqueous medium I used when I wanted good clarity was PVP glycerol. I have no reference, nor do I know who introduced it, but the recipe is:

Polyvinylpyrrolidone 50 grams
Distilled water 50 ml

Stir the two together, and put in a 56C incubator until it is a clear syrup. Add 10 mL glycerol, mix very well. Leave another hour or so for bubbles to rise, then cool to room temperature. In a capped container it will last for years.

Almost dry sections, place the medium onto the center of the section, not the edge of the slide. Apply coverslip. Do not press. Make any adjustments to the coverslip immediately as it sets in about five minutes. Its disadvantage is that it slowly dries back over some months.

I used this medium for muscle biopsy enzymes and it worked very well.

Bryan Llewellyn
----- Original Message -----
From: David Laidley
To: ***@lists.utsouthwestern.edu
Sent: Tuesday, December 02, 2003 9:20 AM
Subject: [Histonet] aqueous mounting medium nad refractive index


Does anyone know the refractive index of Faramount (Dako) and PVA-glycerol, both aqueous mounting mediums.

Can anyone suggest an aqueous mounting medium that has a better refractive index than the ones mentioned above (if available)

David Laidley
Memorial University of Newfoundland
Division of Basic Medical Sciences
St. John's, Newfoundland

***@yahoo.ca