[Histonet] unstained paraffin tissue slides storage

[Pop Elena]

Hello,
I found here a few disscussions regarding the storage of tissue slides but I did
not find a clear answer to the questions I have. I would really appreciate an
answer from anybody that has experience with this.

I need to store for long term a bunch of unstained tissue slides for the purpose
of doing immunostaining even in a few years from now on. Unfortunatelly they
were stored for about 3 years at room temperature. What it is
usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to
store them in the regular 100 slides boxes? And when you need to start an
immunostaining just take them out of the freezer and let them at room temp for a
while before starting the stain or what procedure do you use?

I heard some labs keep them in nitrogen gas containers. Do you have any info
about this?

Any imput is appreciated!
Thanks!

[s***@xbiotech.com]

Plain slide boxes is ok. I think you can store them for up to a year in
a fridg. (4 degrees), but I usually pu them in a freezer (-20).

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas 78744

[phone]




-------- Original Message --------
Subject: [Histonet] unstained paraffin tissue slides storage
From: Pop Elena <***@yahoo.com>
Date: Wed, November 03, 2010 1:21 pm
To: ***@lists.utsouthwestern.edu

Hello,
I found here a few disscussions regarding the storage of tissue slides
but I did
not find a clear answer to the questions I have. I would really
appreciate an
answer from anybody that has experience with this.

I need to store for long term a bunch of unstained tissue slides for the
purpose
of doing immunostaining even in a few years from now on. Unfortunatelly
they
were stored for about 3 years at room temperature. What it is
usually recomended: to store them at -20 degrees Celsius? If yes, is it
OK to
store them in the regular 100 slides boxes? And when you need to start
an
immunostaining just take them out of the freezer and let them at room
temp for a
while before starting the stain or what procedure do you use?

I heard some labs keep them in nitrogen gas containers. Do you have any
info
about this?

Any imput is appreciated!
Thanks!

[Helen Fedor]

Hello, We have been storing our slides in very small Ziploc bags at -20 and find that this method works fairly well. We have done a study(Berez et.al in process) and slides stored in this fashion for 5 years stain better than freshly cut slides from the same blocks that have been stored at room temperature.


Helen L. Fedor

Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660





-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of ***@xbiotech.com
Sent: Wednesday, November 03, 2010 4:26 PM
To: Pop Elena
Cc: ***@lists.utsouthwestern.edu
Subject: RE: [Histonet] unstained paraffin tissue slides storage

Plain slide boxes is ok. I think you can store them for up to a year in
a fridg. (4 degrees), but I usually pu them in a freezer (-20).

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas 78744

[phone]




-------- Original Message --------
Subject: [Histonet] unstained paraffin tissue slides storage
From: Pop Elena <***@yahoo.com>
Date: Wed, November 03, 2010 1:21 pm
To: ***@lists.utsouthwestern.edu

Hello,
I found here a few disscussions regarding the storage of tissue slides
but I did
not find a clear answer to the questions I have. I would really
appreciate an
answer from anybody that has experience with this.

I need to store for long term a bunch of unstained tissue slides for the
purpose
of doing immunostaining even in a few years from now on. Unfortunatelly
they
were stored for about 3 years at room temperature. What it is
usually recomended: to store them at -20 degrees Celsius? If yes, is it
OK to
store them in the regular 100 slides boxes? And when you need to start
an
immunostaining just take them out of the freezer and let them at room
temp for a
while before starting the stain or what procedure do you use?

I heard some labs keep them in nitrogen gas containers. Do you have any
info
about this?

Any imput is appreciated!
Thanks!



_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.

[Edwards, Richard E.]

Room temperature storage has always worked fine for us, of course it all depends on the quality of fixation and processing, and what antigen you are after..........

-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: 03 November 2010 20:55
To: ***@xbiotech.com; Pop Elena
Cc: ***@lists.utsouthwestern.edu
Subject: RE: [Histonet] unstained paraffin tissue slides storage

Hello, We have been storing our slides in very small Ziploc bags at -20 and find that this method works fairly well. We have done a study(Berez et.al in process) and slides stored in this fashion for 5 years stain better than freshly cut slides from the same blocks that have been stored at room temperature.


Helen L. Fedor

Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660





-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu [mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of ***@xbiotech.com
Sent: Wednesday, November 03, 2010 4:26 PM
To: Pop Elena
Cc: ***@lists.utsouthwestern.edu
Subject: RE: [Histonet] unstained paraffin tissue slides storage

Plain slide boxes is ok. I think you can store them for up to a year in a fridg. (4 degrees), but I usually pu them in a freezer (-20).

Sarah Goebel, B.A., HT (ASCP)
Histotechnician


XBiotech USA Inc.

8201 East Riverside Dr. Bldg 4 Suite 100

Austin, Texas 78744

[phone]




-------- Original Message --------
Subject: [Histonet] unstained paraffin tissue slides storage
From: Pop Elena <***@yahoo.com>
Date: Wed, November 03, 2010 1:21 pm
To: ***@lists.utsouthwestern.edu

Hello,
I found here a few disscussions regarding the storage of tissue slides but I did not find a clear answer to the questions I have. I would really appreciate an answer from anybody that has experience with this.

I need to store for long term a bunch of unstained tissue slides for the purpose of doing immunostaining even in a few years from now on. Unfortunatelly they were stored for about 3 years at room temperature. What it is usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to store them in the regular 100 slides boxes? And when you need to start an immunostaining just take them out of the freezer and let them at room temp for a while before starting the stain or what procedure do you use?

I heard some labs keep them in nitrogen gas containers. Do you have any info about this?

Any imput is appreciated!
Thanks!



_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
ht

[Sherwood, Margaret]

We always stored our unstained paraffin slides in slides boxes at -80 degrees.

-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of Pop Elena
Sent: Wednesday, November 03, 2010 4:22 PM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] unstained paraffin tissue slides storage

Hello,
I found here a few disscussions regarding the storage of tissue slides but I did

not find a clear answer to the questions I have. I would really appreciate an
answer from anybody that has experience with this.

I need to store for long term a bunch of unstained tissue slides for the purpose

of doing immunostaining even in a few years from now on. Unfortunatelly they
were stored for about 3 years at room temperature. What it is
usually recomended: to store them at -20 degrees Celsius? If yes, is it OK to
store them in the regular 100 slides boxes? And when you need to start an
immunostaining just take them out of the freezer and let them at room temp for a

while before starting the stain or what procedure do you use?

I heard some labs keep them in nitrogen gas containers. Do you have any info
about this?

Any imput is appreciated!
Thanks!



_______________________________________________
Histonet mailing list
***@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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