[Histonet] subbing positively charged slides

Discussion:

Travers, Susan

2015-07-09 12:45:48 UTC

Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide...

Susan Travers
Professor, Biosciences
Division of Biosciences, College of Dentistry
The Ohio State University
305 West 12th Avenue
Columbus, Ohio 43210
***@osu.edu<mailto:***@osu.edu>
(614)361-0800 (cell)
(614)292-6366 (work)

Geoff

2015-07-09 14:07:00 UTC

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Why would you want to do this?
You could buy plain slides and sub them yourself.

Geoff

Post by Travers, Susan
Does anyone know if it's ok to sub positively charged slides with chrome alum-gelatin. I seem to remember an incident where this did not work- but maybe it's just superstition... it seems as if the chrome alum might be positively charged itself and thus repelled from the surface of the slide...
Susan Travers
Professor, Biosciences
Division of Biosciences, College of Dentistry
The Ohio State University
305 West 12th Avenue
Columbus, Ohio 43210
(614)361-0800 (cell)
(614)292-6366 (work)
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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John Kiernan

2015-07-09 14:12:49 UTC

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Chrome-gelatin cannot be expected to stick well to a glass surface that has been modified by adding aminopropyl (or similar) groups to its silicate ions. See http://publish.uwo.ca/~jkiernan/adhesivs.htm.

John Kiernan
London, Canada
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