[Histonet] Paraffin Embedding of Mice Tissue

[Kelly Salceies]

Hi Everyone,

I am a brand new Tech and am having trouble preping some mouse tissue
for paraffin embedding. I have all different tissues (liver, heart,
quads, brain, lung, and gastroc) and would like to prep all tissues (if
possible) under the same conditions. I have been using the Shandon
Hypercenter XP or the Shandon Histowave (microwave tissue processor...).
Does anybody have a good protocol for mouse tissues on either of these
instruments?? I have tried a number of protocols, but all my tissue has
been really dry in cutting...

Any suggestions would be greatly appreciated!!


Thanks,

Kelly Salceies
University of New Mexico
Health Sciences Center
Dept. of Pathology

[Sarah Jones]

Dear Kelly,
Without having a clue as to what you are doing, I will bet that you
are overprocessing them. Try a 15-20 minute per station program on the
XP and see how they cut. You will see what is over processed and what is
underprocessed and then trim accordingly if you want to run under the
same program. (Mouse spleen and liver can be trimmed in a bit bigger
than others) Sarah

Sarah Jones HT(ASCP)
Dept. of Vet. Anatomy & Public Health
Histology Lab
Texas A&M University
College Station, TX 77843-4458
phone: [phone]
fax: [phone]
Hi Everyone,

I am a brand new Tech and am having trouble preping some mouse tissue
for paraffin embedding. I have all different tissues (liver, heart,
quads, brain, lung, and gastroc) and would like to prep all tissues
(if
possible) under the same conditions. I have been using the Shandon
Hypercenter XP or the Shandon Histowave (microwave tissue
processor...).
Does anybody have a good protocol for mouse tissues on either of these
instruments?? I have tried a number of protocols, but all my tissue
has
been really dry in cutting...

Any suggestions would be greatly appreciated!!


Thanks,

Kelly Salceies
University of New Mexico
Health Sciences Center
Dept. of Pathology

[Katri Tuomala]

Hi Kelly,

What is your fixative for these mice tissues and how long? How big (read
thick) are your sections to be processed?
These two things will determine your routine processing protocol.
I'm not familiar with microwave processing, so I won't comment on that.
If the tissue is well fixed, there really isn't a chance to "over process"
it. The problems arise with inadequate fixation, which then leads to
alcohols and xylene drying out the tissue, and hot paraffin then causes
further damage.
Just something to think about....

Katri

Katri Tuomala
Anatomic Pathology
St.Joseph's Health Care
Hamilton, Ontaorio, Canada


----- Original Message -----
From: "Kelly Salceies" <***@salud.unm.edu>
To: <***@lists.utsouthwestern.edu>
Sent: Monday, September 29, 2003 10:01 AM
Subject: [Histonet] Paraffin Embedding of Mice Tissue

Post by Kelly Salceies
Hi Everyone,
I am a brand new Tech and am having trouble preping some mouse tissue
for paraffin embedding. I have all different tissues (liver, heart,
quads, brain, lung, and gastroc) and would like to prep all tissues (if
possible) under the same conditions. I have been using the Shandon
Hypercenter XP or the Shandon Histowave (microwave tissue processor...).
Does anybody have a good protocol for mouse tissues on either of these
instruments?? I have tried a number of protocols, but all my tissue has
been really dry in cutting...
Any suggestions would be greatly appreciated!!
Thanks,
Kelly Salceies
University of New Mexico
Health Sciences Center
Dept. of Pathology
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