[Histonet] "MBS" in Luxol Fast Blue MBS

[m***@nmr.mgh.harvard.edu]

Hi, I had tried sending this message upon joining
histonet, but I didn't see it in the archive. That
could be why I haven't gotten a reply yet! Here it
is again:

Hi, I've been using luxol fast blue MBS for myelin
staining and would like to know what the acronym "MBS"
represents.

I have read many primary sources, such as articles by
Kluver and Barrera as well as Salthouse, and I have
performed internet searches with Google, all to no
avail (other than finding histonet!).

Thanks for your help!
Megan
=-)

p.s. Also, any explanations of the other acronyms used
in luxol dyes (ARN, G and the "N" in MBSN) would
be much appreciated!

[Bartlett, Jeanine]

Luxol fast blue MBSN is the chemical description for the specific dye,
Solvent Blue 38
C32H12CuN8Na2O6S2
F.W. 732.16


Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA 30333
[phone]
***@cdc.hhs.gov


-----Original Message-----
From: histonet-***@lists.utsouthwestern.edu
[mailto:histonet-***@lists.utsouthwestern.edu] On Behalf Of
***@nmr.mgh.harvard.edu
Sent: Monday, February 06, 2006 1:47 PM
To: ***@lists.utsouthwestern.edu
Subject: [Histonet] "MBS" in Luxol Fast Blue MBS

Hi, I had tried sending this message upon joining histonet, but I didn't
see it in the archive. That could be why I haven't gotten a reply yet!
Here it is again:

Hi, I've been using luxol fast blue MBS for myelin staining and would
like to know what the acronym "MBS"
represents.

I have read many primary sources, such as articles by Kluver and Barrera
as well as Salthouse, and I have performed internet searches with
Google, all to no avail (other than finding histonet!).

Thanks for your help!
Megan
=-)

p.s. Also, any explanations of the other acronyms used
in luxol dyes (ARN, G and the "N" in MBSN) would
be much appreciated!

[John Kiernan]

Your message appeared all right, but nobody
answered. It probably means nobody knows what the
MBS stands for. Luxol fast blues are not a
chemical group of dyes. They are arylguanidinium
salts of anionic dyes (rather than the usual
sodium salts). This makes the dyes soluble in
alcohol but not in water. When sections are
stained, the organic cation stays in the alcohol,
and the coloured anion lodges in the rather
myelin. Like any other anionic dye, a luxol can be
differentiated by alkali. Luxol fast blue MBS is a
phthalocyanine dye; luxol fast blues G and ARN are
azo dyes. See Clasen et al. 1973 J. Neuropath.
Exp. Neurol. 32:271-283 for more on this subject,
including how to make your own luxol-type dyes.

John Kiernan
London, Canada
-----------------------------

Post by m***@nmr.mgh.harvard.edu
Hi, I had tried sending this message upon joining
histonet, but I didn't see it in the archive. That
could be why I haven't gotten a reply yet! Here it
Hi, I've been using luxol fast blue MBS for myelin
staining and would like to know what the acronym "MBS"
represents.
I have read many primary sources, such as articles by
Kluver and Barrera as well as Salthouse, and I have
performed internet searches with Google, all to no
avail (other than finding histonet!).
Thanks for your help!
Megan
=-)
p.s. Also, any explanations of the other acronyms used
in luxol dyes (ARN, G and the "N" in MBSN) would
be much appreciated!
_______________________________________________
Histonet mailing list
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Cheryl]

Hey Fellow Histonetters:
I read all the postings on here and generally understand all of it, but the more I read the more I realize I've forgotten more than I probably remember. (I haven't done a muscle stain group in quite a while!!). Does anyone else feel this way??? (Yes, I've passed 40, I'm sure someone out there can explain the mechanism of memory loss for me!!!)

Thanks for slowing down the brain drain!!

Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one tech at a time.
***@fullstaff.org
281.852.9457 office
281.883.7704 cell

[Cheryl]

Hey Fellow Histonetters:
I read all the postings on here and generally understand all of it, but the more I read the more I realize I've forgotten more than I probably remember. (I haven't done a muscle stain group in quite a while!!). Does anyone else feel this way??? (Yes, I've passed 40, I'm sure someone out there can explain the mechanism of memory loss for me!!!)

Thanks for slowing down the brain drain!!

Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one tech at a time.
***@fullstaff.org
281.852.9457 office
281.883.7704 cell

[Kemlo Rogerson]

I've rationalised from the following standpoint;

When you are younger you usually have less to think about and therefore you
can focus your thoughts better. As you get older you have more to think
about and usually less time and your thoughts become unfocused. I mean my
son arranged perfectly easily to organise his snow boarding holiday but
fails regularly to pay his rent.

I think when you get older you also care more about forgetting; damn where
are my glasses!!!

Kemlo Rogerson
Pathology Manager
Ext 3311
DD 01934 647057
Mob [phone]




-----Original Message-----
From: Cheryl [mailto:***@yahoo.com]
Sent: Tuesday, February 07, 2006 12:02 AM
To: ***@lists.utsouthwestern.edu
Cc: ***@lists.utsouthwestern.edu
Subject: [Histonet] Can anyone explain or at least empathise?

Hey Fellow Histonetters:
I read all the postings on here and generally understand all of it, but
the more I read the more I realize I've forgotten more than I probably
remember. (I haven't done a muscle stain group in quite a while!!). Does
anyone else feel this way??? (Yes, I've passed 40, I'm sure someone out
there can explain the mechanism of memory loss for me!!!)

Thanks for slowing down the brain drain!!

Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one tech at a time.
***@fullstaff.org
281.852.9457 office
281.883.7704 cell